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dna binding buffer  (Zymo Research)


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    Zymo Research dna binding buffer
    Dna Binding Buffer, supplied by Zymo Research, used in various techniques. Bioz Stars score: 96/100, based on 311 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/binding+buffer/DNA+Binding+Buffer/pmc13682663-367-8-21
    Average 96 stars, based on 311 article reviews
    dna binding buffer - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Suspension:

    Article Title: Rat Glioma 101.8 Tissue Strain: Molecular and Morphological Features
    Article Snippet: The cells were counted using acridine orange and propidium iodide (Logos biosystems, Seoul, Korea) on a Luna II cell counter (Logos biosystems, Seoul, Korea). .. The cell suspension was incubated for 15 min; then the volume of the mixtures was adjusted to 500 μL using a binding buffer (Zymo research, Irvine, CA, USA). .. To increase the percentage of cell survival, a dead cell removal kit (Miltenyi biotec, Vianen, The Netherlands) was used.

    Article Title: Rat Glioma 101.8 Tissue Strain: Molecular and Morphological Features.
    Article Snippet: To increase the percentage of cell survival, a dead cell removal kit (Miltenyi biotec, Vianen, The Netherlands) was used. .. The cell suspension was incubated for 15 min; then the volume of the mixtures was adjusted to 500 μL using a binding buffer (Zymo research, Irvine, CA, USA). .. The cells were centrifuged at 300 g; the supernatant fluid was removed, and Gl1 and Gl2 cell samples were resuspended in 300 and 200 μL of Dead Cell Removal microbeads (Miltenyi biotec, Vianen, Netherlands), respectively.

    Incubation:

    Article Title: Rat Glioma 101.8 Tissue Strain: Molecular and Morphological Features
    Article Snippet: The cells were counted using acridine orange and propidium iodide (Logos biosystems, Seoul, Korea) on a Luna II cell counter (Logos biosystems, Seoul, Korea). .. The cell suspension was incubated for 15 min; then the volume of the mixtures was adjusted to 500 μL using a binding buffer (Zymo research, Irvine, CA, USA). .. To increase the percentage of cell survival, a dead cell removal kit (Miltenyi biotec, Vianen, The Netherlands) was used.

    Article Title: Rat Glioma 101.8 Tissue Strain: Molecular and Morphological Features.
    Article Snippet: To increase the percentage of cell survival, a dead cell removal kit (Miltenyi biotec, Vianen, The Netherlands) was used. .. The cell suspension was incubated for 15 min; then the volume of the mixtures was adjusted to 500 μL using a binding buffer (Zymo research, Irvine, CA, USA). .. The cells were centrifuged at 300 g; the supernatant fluid was removed, and Gl1 and Gl2 cell samples were resuspended in 300 and 200 μL of Dead Cell Removal microbeads (Miltenyi biotec, Vianen, Netherlands), respectively.

    Binding Assay:

    Article Title: Rat Glioma 101.8 Tissue Strain: Molecular and Morphological Features
    Article Snippet: The cells were counted using acridine orange and propidium iodide (Logos biosystems, Seoul, Korea) on a Luna II cell counter (Logos biosystems, Seoul, Korea). .. The cell suspension was incubated for 15 min; then the volume of the mixtures was adjusted to 500 μL using a binding buffer (Zymo research, Irvine, CA, USA). .. To increase the percentage of cell survival, a dead cell removal kit (Miltenyi biotec, Vianen, The Netherlands) was used.

    Article Title: Touching Surfaces - Presence of microorganisms on antimicrobial metal surfaces on the International Space Station and in German schools.
    Article Snippet: .. DNA extraction was performed using ZymoBIOMICS DNA microprep kit (Zymo Research, Germany) kit according to the manufacturer’s manual with following modifications: After lysis of cells, supernatant was directly mixed with binding buffer and subsequently transferred to Zymo-Spin IC columns. .. DNA extraction was performed using ZymoBIOMICS DNA microprep kit (Zymo Research, Germany) kit according to the manufacturer’s manual with following modifications: After lysis of cells, supernatant was directly mixed with binding buffer and subsequently transferred to Zymo-Spin IC columns.

    Article Title: Allelic Variation of Helicobacter pylori vacA Gene and Its Association with Gastric Pathologies in Clinical Samples Collected in Jordan
    Article Snippet: .. Next, 400 μL of genomic binding buffer was added and mixed gently for 15 s. The mixture was then transferred to a Zymo-SpinTM IIC-XLR column placed in a collection tube and centrifuged at 12,000× g for 1 min. ..

    Article Title: Rat Glioma 101.8 Tissue Strain: Molecular and Morphological Features.
    Article Snippet: To increase the percentage of cell survival, a dead cell removal kit (Miltenyi biotec, Vianen, The Netherlands) was used. .. The cell suspension was incubated for 15 min; then the volume of the mixtures was adjusted to 500 μL using a binding buffer (Zymo research, Irvine, CA, USA). .. The cells were centrifuged at 300 g; the supernatant fluid was removed, and Gl1 and Gl2 cell samples were resuspended in 300 and 200 μL of Dead Cell Removal microbeads (Miltenyi biotec, Vianen, Netherlands), respectively.

    Article Title: PREVALENCE OF <i>SALMONELLA ENTERICA</i> AND <i>VIBRIO CHOLERAE</i> IN DIARRHOEIC STOOL SAMPLES OF PATIENTS ATTENDING GENERAL HOSPITAL, MINNA, NIGER STATE, NIGERIA
    Article Snippet: .. The lysate was mixed with genomic binding buffer, loaded onto a Zymo spin column, and centrifuged. ..

    Article Title: Individual Staphylococcus aureus SauUSI restriction endonuclease motors fragment methylated DNA
    Article Snippet: .. In brief, SauUSI reactions were quenched in Binding Buffer from the Clean and Concentrate kit (Zymo Research) and purified. .. Samples were treated with Ultra II end repair mix (NEB).

    Article Title: Genome‐Wide Association Study (GWAS) for Linear Body Measurements of Sussex Cattle ( Bos taurus ) at Weaning and Yearling Age in South Africa
    Article Snippet: The DNA was obtained using the Zymo Research Quick‐DNA Miniprep Plus Kit (Zymo Research, USA) following the manufacturer's protocol and quantified using the Qubit dsDNA HS assay kit (Thermo Fisher Scientific). .. Briefly, ∼25 hair root samples were placed in a micro‐centrifuge tube and a solution of 95 μL water, 95 μL solid tissue buffer and 10 μL proteinase K was added before mixing and incubating at 55°C for 1–3 h. Two volumes of genomic binding buffer were then added to the supernatant and mixed for 15 s. The mixture was transferred to a Zymo‐Spin llC‐XLR column in a collection tube and centrifuged at ≥ 12,000 × g for 1 min four times. ..

    DNA Extraction:

    Article Title: Touching Surfaces - Presence of microorganisms on antimicrobial metal surfaces on the International Space Station and in German schools.
    Article Snippet: .. DNA extraction was performed using ZymoBIOMICS DNA microprep kit (Zymo Research, Germany) kit according to the manufacturer’s manual with following modifications: After lysis of cells, supernatant was directly mixed with binding buffer and subsequently transferred to Zymo-Spin IC columns. .. DNA extraction was performed using ZymoBIOMICS DNA microprep kit (Zymo Research, Germany) kit according to the manufacturer’s manual with following modifications: After lysis of cells, supernatant was directly mixed with binding buffer and subsequently transferred to Zymo-Spin IC columns.

    Article Title: Touching Surfaces – Presence of microorganisms on antimicrobial metal surfaces on the International Space Station and in German schools
    Article Snippet: .. DNA extraction was performed using ZymoBIOMICS DNA microprep kit ( Zymo Research , Germany ) kit according to the manufacturer’s manual with following modifications: After lysis of cells, supernatant was directly mixed with binding buffer and subsequently transferred to Zymo-Spin IC columns. ..

    Lysis:

    Article Title: Touching Surfaces - Presence of microorganisms on antimicrobial metal surfaces on the International Space Station and in German schools.
    Article Snippet: .. DNA extraction was performed using ZymoBIOMICS DNA microprep kit (Zymo Research, Germany) kit according to the manufacturer’s manual with following modifications: After lysis of cells, supernatant was directly mixed with binding buffer and subsequently transferred to Zymo-Spin IC columns. .. DNA extraction was performed using ZymoBIOMICS DNA microprep kit (Zymo Research, Germany) kit according to the manufacturer’s manual with following modifications: After lysis of cells, supernatant was directly mixed with binding buffer and subsequently transferred to Zymo-Spin IC columns.

    Article Title: Touching Surfaces – Presence of microorganisms on antimicrobial metal surfaces on the International Space Station and in German schools
    Article Snippet: .. DNA extraction was performed using ZymoBIOMICS DNA microprep kit ( Zymo Research , Germany ) kit according to the manufacturer’s manual with following modifications: After lysis of cells, supernatant was directly mixed with binding buffer and subsequently transferred to Zymo-Spin IC columns. ..

    Purification:

    Article Title: Individual Staphylococcus aureus SauUSI restriction endonuclease motors fragment methylated DNA
    Article Snippet: .. In brief, SauUSI reactions were quenched in Binding Buffer from the Clean and Concentrate kit (Zymo Research) and purified. .. Samples were treated with Ultra II end repair mix (NEB).



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    ( A ) Immunoblotting of SIINFEKL peptide–pulsed Neuro2a cell lines cocultured with activated OT-I T cells for 4 hours at ratios of T cells to target cells indicated. ( B ) Schematic representation of fluorescence-activated cell sorting (FACS) based <t>on</t> <t>Caspase-3(DEVD)NucView</t> and <t>annexin</t> V (AnnV) staining, applies to (C) to (E). Created in BioRender; B. Liu (2026), https://BioRender.com/y3nlv0g . ( C ) Rate of outgrowth from cells recovered from FACS sort, calculated on the basis of number of initially plated cells. Data were compiled from five independent experiments. Paired t test. ( D and E ) Quantification of nucleotide fragments with greater than three, four, five, or six single-nucleotide variants (SNVs) from various recovered populations as indicated. ( F ) The design of the ATM–fluorescence resonance energy transfer (FRET) reporter. Created in BioRender; B. Liu (2026), https://BioRender.com/jqdhdx7 . ( G ) Quantification of FRET loss signal from Neuro2a expressing the ATM-FRET reporter shown in (F). Neuro2a cells were SIINFEKL peptide pulsed and then coincubated with OT-I T cells for 6 hours at 10:1 T cell–to–target cell ratio. Data were pooled from three independent experiments. Unpaired t test. ( H ) In vivo growth of Neuro2a cells expressing the ATM-FRET sensor. ATM-FRET loss populations were sorted post BH3m treatment (1 μM ABT737 and 1 μM S63845 ). Cells were sorted for FRET-loss population at 2.5 hours, and 1 × 10 4 cells were injected into NSG mice ( n = 5 animals per group). Kaplan-Meyer survival curves. WT-base and GE KO–base refer to ATM-FRET–expressing cells that were not treated with BH3m. Log-rank (Mantel-Cox) test. * P < 0.05 and **** P < 0.0001. C3, active caspase-3.
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    ( A ) Immunoblotting of SIINFEKL peptide–pulsed Neuro2a cell lines cocultured with activated OT-I T cells for 4 hours at ratios of T cells to target cells indicated. ( B ) Schematic representation of fluorescence-activated cell sorting (FACS) based <t>on</t> <t>Caspase-3(DEVD)NucView</t> and <t>annexin</t> V (AnnV) staining, applies to (C) to (E). Created in BioRender; B. Liu (2026), https://BioRender.com/y3nlv0g . ( C ) Rate of outgrowth from cells recovered from FACS sort, calculated on the basis of number of initially plated cells. Data were compiled from five independent experiments. Paired t test. ( D and E ) Quantification of nucleotide fragments with greater than three, four, five, or six single-nucleotide variants (SNVs) from various recovered populations as indicated. ( F ) The design of the ATM–fluorescence resonance energy transfer (FRET) reporter. Created in BioRender; B. Liu (2026), https://BioRender.com/jqdhdx7 . ( G ) Quantification of FRET loss signal from Neuro2a expressing the ATM-FRET reporter shown in (F). Neuro2a cells were SIINFEKL peptide pulsed and then coincubated with OT-I T cells for 6 hours at 10:1 T cell–to–target cell ratio. Data were pooled from three independent experiments. Unpaired t test. ( H ) In vivo growth of Neuro2a cells expressing the ATM-FRET sensor. ATM-FRET loss populations were sorted post BH3m treatment (1 μM ABT737 and 1 μM S63845 ). Cells were sorted for FRET-loss population at 2.5 hours, and 1 × 10 4 cells were injected into NSG mice ( n = 5 animals per group). Kaplan-Meyer survival curves. WT-base and GE KO–base refer to ATM-FRET–expressing cells that were not treated with BH3m. Log-rank (Mantel-Cox) test. * P < 0.05 and **** P < 0.0001. C3, active caspase-3.
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    ( A ) Immunoblotting of SIINFEKL peptide–pulsed Neuro2a cell lines cocultured with activated OT-I T cells for 4 hours at ratios of T cells to target cells indicated. ( B ) Schematic representation of fluorescence-activated cell sorting (FACS) based on Caspase-3(DEVD)NucView and annexin V (AnnV) staining, applies to (C) to (E). Created in BioRender; B. Liu (2026), https://BioRender.com/y3nlv0g . ( C ) Rate of outgrowth from cells recovered from FACS sort, calculated on the basis of number of initially plated cells. Data were compiled from five independent experiments. Paired t test. ( D and E ) Quantification of nucleotide fragments with greater than three, four, five, or six single-nucleotide variants (SNVs) from various recovered populations as indicated. ( F ) The design of the ATM–fluorescence resonance energy transfer (FRET) reporter. Created in BioRender; B. Liu (2026), https://BioRender.com/jqdhdx7 . ( G ) Quantification of FRET loss signal from Neuro2a expressing the ATM-FRET reporter shown in (F). Neuro2a cells were SIINFEKL peptide pulsed and then coincubated with OT-I T cells for 6 hours at 10:1 T cell–to–target cell ratio. Data were pooled from three independent experiments. Unpaired t test. ( H ) In vivo growth of Neuro2a cells expressing the ATM-FRET sensor. ATM-FRET loss populations were sorted post BH3m treatment (1 μM ABT737 and 1 μM S63845 ). Cells were sorted for FRET-loss population at 2.5 hours, and 1 × 10 4 cells were injected into NSG mice ( n = 5 animals per group). Kaplan-Meyer survival curves. WT-base and GE KO–base refer to ATM-FRET–expressing cells that were not treated with BH3m. Log-rank (Mantel-Cox) test. * P < 0.05 and **** P < 0.0001. C3, active caspase-3.

    Journal: Science Advances

    Article Title: Tumor gasdermin E acts as a rheostat to limit apoptotic cell survival and mutagenesis in anticancer immune environments

    doi: 10.1126/sciadv.aeb1137

    Figure Lengend Snippet: ( A ) Immunoblotting of SIINFEKL peptide–pulsed Neuro2a cell lines cocultured with activated OT-I T cells for 4 hours at ratios of T cells to target cells indicated. ( B ) Schematic representation of fluorescence-activated cell sorting (FACS) based on Caspase-3(DEVD)NucView and annexin V (AnnV) staining, applies to (C) to (E). Created in BioRender; B. Liu (2026), https://BioRender.com/y3nlv0g . ( C ) Rate of outgrowth from cells recovered from FACS sort, calculated on the basis of number of initially plated cells. Data were compiled from five independent experiments. Paired t test. ( D and E ) Quantification of nucleotide fragments with greater than three, four, five, or six single-nucleotide variants (SNVs) from various recovered populations as indicated. ( F ) The design of the ATM–fluorescence resonance energy transfer (FRET) reporter. Created in BioRender; B. Liu (2026), https://BioRender.com/jqdhdx7 . ( G ) Quantification of FRET loss signal from Neuro2a expressing the ATM-FRET reporter shown in (F). Neuro2a cells were SIINFEKL peptide pulsed and then coincubated with OT-I T cells for 6 hours at 10:1 T cell–to–target cell ratio. Data were pooled from three independent experiments. Unpaired t test. ( H ) In vivo growth of Neuro2a cells expressing the ATM-FRET sensor. ATM-FRET loss populations were sorted post BH3m treatment (1 μM ABT737 and 1 μM S63845 ). Cells were sorted for FRET-loss population at 2.5 hours, and 1 × 10 4 cells were injected into NSG mice ( n = 5 animals per group). Kaplan-Meyer survival curves. WT-base and GE KO–base refer to ATM-FRET–expressing cells that were not treated with BH3m. Log-rank (Mantel-Cox) test. * P < 0.05 and **** P < 0.0001. C3, active caspase-3.

    Article Snippet: Cells were spun down and resuspended in DMEM supplemented with annexin binding buffer (99902, Biotium) and stained with annexin V-647 and/or Caspase-3(DEVD)NucView-488, as indicated in the text, for 10 min at 37°C.

    Techniques: Western Blot, Fluorescence, FACS, Staining, Förster Resonance Energy Transfer, Expressing, In Vivo, Injection

    ( A ) Coculture (1:1) of parental B16F10 cells expressing pBabe-Cerulean (blue) and B16F10 cells expressing GsdmD-C3 and mCherry (red) (see movie S1). ( B ) Times of individual cell shrinkage and cell membrane lysis, as in (A). Unpaired t test. ( C ) Apoptotic phase (shrinkage-lysis interval) from (B). One-way ANOVA. ( D ) Quantification of annexin V + SIINFEKL peptide–pulsed B16F10 [as in (B) and (C)] that remains fluorescent when coincubated with activated OT-I T cells at 4 hours. Two-way ANOVA. ( E ) Quantification of SIINFEKL peptide–pulsed B16F10 cell populations with ectopic caspase-3-activatable GSDMD (GD-C3) by fluorescence signal at 24 hours of coincubation with activated OT-I T cells. ( F ) As in (E) with Gsdme. [(E) and (F)] Paired t test. ( G ) Number of GCamp6s spikes observed from SIINFEKL peptide–pulsed Neuro2a cells, coincubated with activated OT-I T cells, 10:1 T cell–to–target cell ratio. Images were taken every 2 min for a duration of 3 hours immediately following the additional of OT-I T cells, and numbers of observable GCamp6s spikes were counted and plotted (see movies S2 to S4 and fig. S5C). Unpaired t test. ( H ) Design for (I) and (J). Created in BioRender; B. Liu (2026), https://BioRender.com/j67ipmw . ( I ) Bioluminescence signal intensity of Neuro2a in C57BL/6 animals ( n = 9 animals per group). Two-way ANOVA. ( J ) Kaplan-Meyer survival curves from C57BL/6 mice injected with Neuro2a cells ( n = 9 animals per group). ( K ) Design for (L). Created in BioRender; B. Liu (2026), https://BioRender.com/j67ipmw . ( L ) Bioluminescence signal intensity of Neuro2a in C57BL/6 animals (control, n = 9 animals; Gsdme −/− , n = 8 animals). ( M ) Kaplan-Meyer survival curves from C57BL/6 mice rechallenged with Neuro2a cells [ n = 5 animals per group; log-rank (Mantel-Cox) test]. ( N ) As in (M) with B16F10 [ n = 12 animals in naïve group, 14 animals in rechallenge group; log-rank (Mantel-Cox) test]. * P < 0.05, ** P < 0.01, and **** P < 0.0001. iv, intravenous; ip, intraperitoneal.

    Journal: Science Advances

    Article Title: Tumor gasdermin E acts as a rheostat to limit apoptotic cell survival and mutagenesis in anticancer immune environments

    doi: 10.1126/sciadv.aeb1137

    Figure Lengend Snippet: ( A ) Coculture (1:1) of parental B16F10 cells expressing pBabe-Cerulean (blue) and B16F10 cells expressing GsdmD-C3 and mCherry (red) (see movie S1). ( B ) Times of individual cell shrinkage and cell membrane lysis, as in (A). Unpaired t test. ( C ) Apoptotic phase (shrinkage-lysis interval) from (B). One-way ANOVA. ( D ) Quantification of annexin V + SIINFEKL peptide–pulsed B16F10 [as in (B) and (C)] that remains fluorescent when coincubated with activated OT-I T cells at 4 hours. Two-way ANOVA. ( E ) Quantification of SIINFEKL peptide–pulsed B16F10 cell populations with ectopic caspase-3-activatable GSDMD (GD-C3) by fluorescence signal at 24 hours of coincubation with activated OT-I T cells. ( F ) As in (E) with Gsdme. [(E) and (F)] Paired t test. ( G ) Number of GCamp6s spikes observed from SIINFEKL peptide–pulsed Neuro2a cells, coincubated with activated OT-I T cells, 10:1 T cell–to–target cell ratio. Images were taken every 2 min for a duration of 3 hours immediately following the additional of OT-I T cells, and numbers of observable GCamp6s spikes were counted and plotted (see movies S2 to S4 and fig. S5C). Unpaired t test. ( H ) Design for (I) and (J). Created in BioRender; B. Liu (2026), https://BioRender.com/j67ipmw . ( I ) Bioluminescence signal intensity of Neuro2a in C57BL/6 animals ( n = 9 animals per group). Two-way ANOVA. ( J ) Kaplan-Meyer survival curves from C57BL/6 mice injected with Neuro2a cells ( n = 9 animals per group). ( K ) Design for (L). Created in BioRender; B. Liu (2026), https://BioRender.com/j67ipmw . ( L ) Bioluminescence signal intensity of Neuro2a in C57BL/6 animals (control, n = 9 animals; Gsdme −/− , n = 8 animals). ( M ) Kaplan-Meyer survival curves from C57BL/6 mice rechallenged with Neuro2a cells [ n = 5 animals per group; log-rank (Mantel-Cox) test]. ( N ) As in (M) with B16F10 [ n = 12 animals in naïve group, 14 animals in rechallenge group; log-rank (Mantel-Cox) test]. * P < 0.05, ** P < 0.01, and **** P < 0.0001. iv, intravenous; ip, intraperitoneal.

    Article Snippet: Cells were spun down and resuspended in DMEM supplemented with annexin binding buffer (99902, Biotium) and stained with annexin V-647 and/or Caspase-3(DEVD)NucView-488, as indicated in the text, for 10 min at 37°C.

    Techniques: Expressing, Membrane, Lysis, Fluorescence, Injection, Control